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plexiglas conditioning chamber noldus phenotyper  (Noldus Information Technology)

 
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    Noldus Information Technology plexiglas conditioning chamber noldus phenotyper
    Heterozygous PME-KO mice are resistant to Aβ-induced impairments in contextual fear <t>conditioning.</t> A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,70) = 4.424, p = 0.0066). Tukey's multiple comparisons show that only the control+Aβ group was significantly different from all other groups (p = 0.0218 vs control+vehicle, p = 0.0367 vs PME-KO+vehicle, and p = 0.0164 vs PME-KO+Aβ*). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,70) = 0.7894, p = 0.5039). N = 16 control+vehicle, 20 control+Aβ, 20 PME-KO+vehicle, and 18 PME-KO+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors shows a significant effect of day (F(1,49) = 41.31, p < 0.0001) but not group (F(3,49) = 2, p = 0.1262). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,49) = 47.28, p < 0.0001) but not group (F(3,49) = 0.5627, p = 0.6422). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,79) = 0.0092, p = 0.9988; for gross motor response: F(3,79) = 1.119, p = 0.3467; for vocalization: F(3,79) = 0.4069, p = 0.7484). N = 16 control+vehicle, 23 control+Aβ, 23 PME+vehicle, and 21 PME+Aβ.
    Plexiglas Conditioning Chamber Noldus Phenotyper, supplied by Noldus Information Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plexiglas+conditioning+chamber+noldus+phenotyper/plexiglas+chamber+noldus+phenotyper+box/pmc07275859-81-8-20
    Average 90 stars, based on 1 article reviews
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    1) Product Images from "Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice"

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.2983-19.2020

    Heterozygous PME-KO mice are resistant to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,70) = 4.424, p = 0.0066). Tukey's multiple comparisons show that only the control+Aβ group was significantly different from all other groups (p = 0.0218 vs control+vehicle, p = 0.0367 vs PME-KO+vehicle, and p = 0.0164 vs PME-KO+Aβ*). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,70) = 0.7894, p = 0.5039). N = 16 control+vehicle, 20 control+Aβ, 20 PME-KO+vehicle, and 18 PME-KO+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors shows a significant effect of day (F(1,49) = 41.31, p < 0.0001) but not group (F(3,49) = 2, p = 0.1262). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,49) = 47.28, p < 0.0001) but not group (F(3,49) = 0.5627, p = 0.6422). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,79) = 0.0092, p = 0.9988; for gross motor response: F(3,79) = 1.119, p = 0.3467; for vocalization: F(3,79) = 0.4069, p = 0.7484). N = 16 control+vehicle, 23 control+Aβ, 23 PME+vehicle, and 21 PME+Aβ.
    Figure Legend Snippet: Heterozygous PME-KO mice are resistant to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,70) = 4.424, p = 0.0066). Tukey's multiple comparisons show that only the control+Aβ group was significantly different from all other groups (p = 0.0218 vs control+vehicle, p = 0.0367 vs PME-KO+vehicle, and p = 0.0164 vs PME-KO+Aβ*). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,70) = 0.7894, p = 0.5039). N = 16 control+vehicle, 20 control+Aβ, 20 PME-KO+vehicle, and 18 PME-KO+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors shows a significant effect of day (F(1,49) = 41.31, p < 0.0001) but not group (F(3,49) = 2, p = 0.1262). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,49) = 47.28, p < 0.0001) but not group (F(3,49) = 0.5627, p = 0.6422). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,79) = 0.0092, p = 0.9988; for gross motor response: F(3,79) = 1.119, p = 0.3467; for vocalization: F(3,79) = 0.4069, p = 0.7484). N = 16 control+vehicle, 23 control+Aβ, 23 PME+vehicle, and 21 PME+Aβ.

    Techniques Used: Control

    Reduced LCMT-1 expression increases sensitivity to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,66) = 5.932, p = 0.0012). Tukey's multiple comparisons show that only the LCMT+Aβ group was significantly different from vehicle-treated controls (*p = 0.0015), and that the LCMT+Aβ group was also significantly different from Aβ-treated controls (p = 0.0272). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,66) = 1.284, p = 0.2873). N = 16 control+vehicle, 16 control+Aβ, 19 LCMT-GT+vehicle, and 19 LCMT-GT+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors showed no significant effect of day (F(1,44) = 1.284, p = 0.2634) or group (F(3,44) = 0.1835, p = 0.9070). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,54) = 7.45, p = 0.0085) but not group (F(3,54) = 0.6784, p = 0.5691). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,74) = 0.5746, p = 0.6335; for gross motor response: F(3,74) = 0.1716, p = 0.91523; for vocalization: F(3,74) = 0.3167, p = 0.8133). N = 18 control+vehicle, 16 control+Aβ, 21 LCMT-GT+vehicle, and 23 LCMT-GT+Aβ.
    Figure Legend Snippet: Reduced LCMT-1 expression increases sensitivity to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,66) = 5.932, p = 0.0012). Tukey's multiple comparisons show that only the LCMT+Aβ group was significantly different from vehicle-treated controls (*p = 0.0015), and that the LCMT+Aβ group was also significantly different from Aβ-treated controls (p = 0.0272). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,66) = 1.284, p = 0.2873). N = 16 control+vehicle, 16 control+Aβ, 19 LCMT-GT+vehicle, and 19 LCMT-GT+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors showed no significant effect of day (F(1,44) = 1.284, p = 0.2634) or group (F(3,44) = 0.1835, p = 0.9070). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,54) = 7.45, p = 0.0085) but not group (F(3,54) = 0.6784, p = 0.5691). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,74) = 0.5746, p = 0.6335; for gross motor response: F(3,74) = 0.1716, p = 0.91523; for vocalization: F(3,74) = 0.3167, p = 0.8133). N = 18 control+vehicle, 16 control+Aβ, 21 LCMT-GT+vehicle, and 23 LCMT-GT+Aβ.

    Techniques Used: Expressing, Control

    Related Articles

    Control:

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice
    Article Snippet: and time required to reach the hidden platform (latency) and swim speed were determined using Ethovision behavioral analysis software (Noldus). .. Contextual fear conditioning Animals were placed into a transparent Plexiglas conditioning chamber (33 cm × 20 cm × 22 cm) (Noldus PhenoTyper). .. Animal movement was recorded using an overhead video camera connected to a personal computer, and freezing behavior was scored and analyzed using Etho

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice
    Article Snippet: Animals were placed into a transparent Plexiglas conditioning chamber (33 cm × 20 cm × 22 cm) (Noldus PhenoTyper). .. A

    Expressing:

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice
    Article Snippet: and time required to reach the hidden platform (latency) and swim speed were determined using Ethovision behavioral analysis software (Noldus). .. Contextual fear conditioning Animals were placed into a transparent Plexiglas conditioning chamber (33 cm × 20 cm × 22 cm) (Noldus PhenoTyper). .. Animal movement was recorded using an overhead video camera connected to a personal computer, and freezing behavior was scored and analyzed using Etho

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice
    Article Snippet: Animals were placed into a transparent Plexiglas conditioning chamber (33 cm × 20 cm × 22 cm) (Noldus PhenoTyper). .. A



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    plexiglas conditioning chamber noldus phenotyper  (Noldus Information Technology)
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    Noldus Information Technology plexiglas conditioning chamber noldus phenotyper
    Heterozygous PME-KO mice are resistant to Aβ-induced impairments in contextual fear <t>conditioning.</t> A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,70) = 4.424, p = 0.0066). Tukey's multiple comparisons show that only the control+Aβ group was significantly different from all other groups (p = 0.0218 vs control+vehicle, p = 0.0367 vs PME-KO+vehicle, and p = 0.0164 vs PME-KO+Aβ*). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,70) = 0.7894, p = 0.5039). N = 16 control+vehicle, 20 control+Aβ, 20 PME-KO+vehicle, and 18 PME-KO+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors shows a significant effect of day (F(1,49) = 41.31, p < 0.0001) but not group (F(3,49) = 2, p = 0.1262). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,49) = 47.28, p < 0.0001) but not group (F(3,49) = 0.5627, p = 0.6422). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,79) = 0.0092, p = 0.9988; for gross motor response: F(3,79) = 1.119, p = 0.3467; for vocalization: F(3,79) = 0.4069, p = 0.7484). N = 16 control+vehicle, 23 control+Aβ, 23 PME+vehicle, and 21 PME+Aβ.
    Plexiglas Conditioning Chamber Noldus Phenotyper, supplied by Noldus Information Technology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plexiglas+conditioning+chamber+noldus+phenotyper/plexiglas+chamber+noldus+phenotyper+box/pmc07275859-81-8-20
    Average 90 stars, based on 1 article reviews
    plexiglas conditioning chamber noldus phenotyper - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    Heterozygous PME-KO mice are resistant to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,70) = 4.424, p = 0.0066). Tukey's multiple comparisons show that only the control+Aβ group was significantly different from all other groups (p = 0.0218 vs control+vehicle, p = 0.0367 vs PME-KO+vehicle, and p = 0.0164 vs PME-KO+Aβ*). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,70) = 0.7894, p = 0.5039). N = 16 control+vehicle, 20 control+Aβ, 20 PME-KO+vehicle, and 18 PME-KO+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors shows a significant effect of day (F(1,49) = 41.31, p < 0.0001) but not group (F(3,49) = 2, p = 0.1262). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,49) = 47.28, p < 0.0001) but not group (F(3,49) = 0.5627, p = 0.6422). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,79) = 0.0092, p = 0.9988; for gross motor response: F(3,79) = 1.119, p = 0.3467; for vocalization: F(3,79) = 0.4069, p = 0.7484). N = 16 control+vehicle, 23 control+Aβ, 23 PME+vehicle, and 21 PME+Aβ.

    Journal: The Journal of Neuroscience

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice

    doi: 10.1523/JNEUROSCI.2983-19.2020

    Figure Lengend Snippet: Heterozygous PME-KO mice are resistant to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,70) = 4.424, p = 0.0066). Tukey's multiple comparisons show that only the control+Aβ group was significantly different from all other groups (p = 0.0218 vs control+vehicle, p = 0.0367 vs PME-KO+vehicle, and p = 0.0164 vs PME-KO+Aβ*). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,70) = 0.7894, p = 0.5039). N = 16 control+vehicle, 20 control+Aβ, 20 PME-KO+vehicle, and 18 PME-KO+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors shows a significant effect of day (F(1,49) = 41.31, p < 0.0001) but not group (F(3,49) = 2, p = 0.1262). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,49) = 47.28, p < 0.0001) but not group (F(3,49) = 0.5627, p = 0.6422). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 16 control+Aβ, 15 PME+vehicle, and 12 PME+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,79) = 0.0092, p = 0.9988; for gross motor response: F(3,79) = 1.119, p = 0.3467; for vocalization: F(3,79) = 0.4069, p = 0.7484). N = 16 control+vehicle, 23 control+Aβ, 23 PME+vehicle, and 21 PME+Aβ.

    Article Snippet: Contextual fear conditioning Animals were placed into a transparent Plexiglas conditioning chamber (33 cm × 20 cm × 22 cm) (Noldus PhenoTyper).

    Techniques: Control

    Reduced LCMT-1 expression increases sensitivity to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,66) = 5.932, p = 0.0012). Tukey's multiple comparisons show that only the LCMT+Aβ group was significantly different from vehicle-treated controls (*p = 0.0015), and that the LCMT+Aβ group was also significantly different from Aβ-treated controls (p = 0.0272). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,66) = 1.284, p = 0.2873). N = 16 control+vehicle, 16 control+Aβ, 19 LCMT-GT+vehicle, and 19 LCMT-GT+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors showed no significant effect of day (F(1,44) = 1.284, p = 0.2634) or group (F(3,44) = 0.1835, p = 0.9070). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,54) = 7.45, p = 0.0085) but not group (F(3,54) = 0.6784, p = 0.5691). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,74) = 0.5746, p = 0.6335; for gross motor response: F(3,74) = 0.1716, p = 0.91523; for vocalization: F(3,74) = 0.3167, p = 0.8133). N = 18 control+vehicle, 16 control+Aβ, 21 LCMT-GT+vehicle, and 23 LCMT-GT+Aβ.

    Journal: The Journal of Neuroscience

    Article Title: Reduced Expression of the PP2A Methylesterase, PME-1, or the PP2A Methyltransferase, LCMT-1, Alters Sensitivity to Beta-Amyloid-Induced Cognitive and Electrophysiological Impairments in Mice

    doi: 10.1523/JNEUROSCI.2983-19.2020

    Figure Lengend Snippet: Reduced LCMT-1 expression increases sensitivity to Aβ-induced impairments in contextual fear conditioning. A, Plot of average percent of time spent freezing (±SEM) during initial exposure to the training context (baseline) and 24 h after footshock for the indicated groups. ANOVA for freezing at 24 h showed a significant difference among groups (F(3,66) = 5.932, p = 0.0012). Tukey's multiple comparisons show that only the LCMT+Aβ group was significantly different from vehicle-treated controls (*p = 0.0015), and that the LCMT+Aβ group was also significantly different from Aβ-treated controls (p = 0.0272). No differences in baseline freezing on day 1 were observed among groups (ANOVA: F(3,66) = 1.284, p = 0.2873). N = 16 control+vehicle, 16 control+Aβ, 19 LCMT-GT+vehicle, and 19 LCMT-GT+Aβ. B, Plot of average distance traveled (±SEM) for the indicated groups during 10 min exposures to an open field environment on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for distance with day and group as factors showed no significant effect of day (F(1,44) = 1.284, p = 0.2634) or group (F(3,44) = 0.1835, p = 0.9070). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. C, Plot of average number of entries into the center of an open field arena (±SEM) for the indicated groups during two 10 min exposures on subsequent days revealed no significant differences between groups. Two-way repeated-measures ANOVA for entries with day and group as factors shows a significant effect of day (F(1,54) = 7.45, p = 0.0085) but not group (F(3,54) = 0.6784, p = 0.5691). Pairwise Bonferroni comparisons between groups within a training day revealed no significant differences with p values < 0.05. N = 10 control+vehicle, 9 control+Aβ, 13 LCMT-GT+vehicle, and 16 LCMT-GT+Aβ. D, Plot of average threshold for responses to footshocks of increasing intensity for the indicated groups revealed no significant differences in footshock perception (ANOVA for visible response: F(3,74) = 0.5746, p = 0.6335; for gross motor response: F(3,74) = 0.1716, p = 0.91523; for vocalization: F(3,74) = 0.3167, p = 0.8133). N = 18 control+vehicle, 16 control+Aβ, 21 LCMT-GT+vehicle, and 23 LCMT-GT+Aβ.

    Article Snippet: Contextual fear conditioning Animals were placed into a transparent Plexiglas conditioning chamber (33 cm × 20 cm × 22 cm) (Noldus PhenoTyper).

    Techniques: Expressing, Control